#
# Please direct questions and support issues to <bioperl-l@bioperl.org>
#
# Cared for by
#
# Copyright to a FUGU Student Intern
#
# You may distribute this module under the same terms as perl itself
# POD documentation - main docs before the code
=head1 NAME
Bio::Tools::Run::Genewise - Object for predicting genes in a
given sequence given a protein
=head1 SYNOPSIS
# Build a Genewise alignment factory
my $factory = Bio::Tools::Run::Genewise->new();
# Pass the factory 2 Bio:SeqI objects (in the order of query peptide
# and target_genomic).
# @genes is an array of Bio::SeqFeature::Gene::GeneStructure objects
my @genes = $factory->run($protein_seq, $genomic_seq);
# Alternatively pass the factory a profile HMM filename and a
# Bio:SeqI object (in the order of query HMM and target_genomic).
# Set hmmer switch first to tell genewise to expect an HMM
$factory->hmmer(1);
my @genes = $factory->run($hmmfile, $genomic_seq);
=head1 DESCRIPTION
Genewise is a gene prediction program developed by Ewan Birney
http://www.sanger.ac.uk/software/wise2.
=head2 Available Params:
NB: These should be passed without the '-' or they will be ignored,
except switches such as 'hmmer' (which have no corresponding value)
which should be set on the factory object using the AUTOLOADed methods
of the same name.
Model [-codon,-gene,-cfreq,-splice,-subs,-indel,-intron,-null]
Alg [-kbyte,-alg]
HMM [-hmmer]
Output [-gff,-gener,-alb,-pal,-block,-divide]
Standard [-help,-version,-silent,-quiet,-errorlog]
=head1 FEEDBACK
=head2 Mailing Lists
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to one
of the Bioperl mailing lists. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/wiki/Mailing_lists - About the mailing lists
=head2 Support
Please direct usage questions or support issues to the mailing list:
I<bioperl-l@bioperl.org>
rather than to the module maintainer directly. Many experienced and
reponsive experts will be able look at the problem and quickly
address it. Please include a thorough description of the problem
with code and data examples if at all possible.
=head2 Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution. Bug reports can be submitted via
the web:
http://redmine.open-bio.org/projects/bioperl/
=head1 AUTHOR - FUGU Student Intern
Email: fugui@worf.fugu-sg.org
=head1 CONTRIBUTORS
Jason Stajich jason-AT-bioperl_DOT_org
Keith James kdj@sanger.ac.uk
=head1 APPENDIX
The rest of the documentation details each of the object
methods. Internal methods are usually preceded with a _
=cut
package Bio::Tools::Run::Genewise;
use vars qw($AUTOLOAD @ISA $PROGRAM $PROGRAMDIR $PROGRAMNAME
@GENEWISE_SWITCHES @GENEWISE_PARAMS
@OTHER_SWITCHES %OK_FIELD);
use Bio::SeqIO;
use Bio::SeqFeature::Generic;
use Bio::SeqFeature::Gene::Exon;
use Bio::Root::Root;
use Bio::Tools::Run::WrapperBase;
use Bio::SeqFeature::FeaturePair;
use Bio::SeqFeature::Gene::Transcript;
use Bio::SeqFeature::Gene::GeneStructure;
use Bio::Tools::Genewise;
use Bio::Tools::AnalysisResult;
use strict;
@ISA = qw(Bio::Root::Root Bio::Tools::Run::WrapperBase );
# Two ways to run the program .....
# 1. define an environmental variable WISEDIR
# export WISEDIR =/usr/local/share/wise2.2.0
# where the wise2.2.20 package is installed
#
# 2. include a definition of an environmental variable WISEDIR in
# every script that will use DBA.pm
# $ENV{WISEDIR} = '/usr/local/share/wise2.2.20';
BEGIN {
@GENEWISE_PARAMS = qw( DYMEM CODON GENE CFREQ SPLICE GENESTATS INIT
SUBS INDEL INTRON NULL INSERT SPLICE_MAX_COLLAR SPLICE_MIN_COLLAR
GW_EDGEQUERY GW_EDGETARGET GW_SPLICESPREAD
KBYTE HNAME ALG BLOCK DIVIDE GENER U V S T G E M);
@GENEWISE_SWITCHES = qw(HELP SILENT QUIET ERROROFFSTD TREV PSEUDO NOSPLICE_GTAG
SPLICE_GTAG NOGWHSP GWHSP
TFOR TABS BOTH HMMER );
# Authorize attribute fields
foreach my $attr ( @GENEWISE_PARAMS, @GENEWISE_SWITCHES,
@OTHER_SWITCHES) { $OK_FIELD{$attr}++; }
}
=head2 program_name
Title : program_name
Usage : $factory>program_name()
Function: holds the program name
Returns: string
Args : None
=cut
sub program_name {
return 'genewise';
}
=head2 program_dir
Title : program_dir
Usage : $factory->program_dir(@params)
Function: returns the program directory, obtained from ENV variable.
Returns: string
Args :
=cut
sub program_dir {
return Bio::Root::IO->catfile($ENV{WISEDIR},"/src/bin/") if $ENV{WISEDIR};
}
sub new {
my ($class, @args) = @_;
my $self = $class->SUPER::new(@args);
my ($attr, $value);
while (@args) {
$attr = shift @args;
$value = shift @args;
next if( $attr =~ /^-/ ); # don't want named parameters
$self->$attr($value);
}
return $self;
}
sub AUTOLOAD {
my $self = shift;
my $attr = $AUTOLOAD;
$attr =~ s/.*:://;
$attr = uc $attr;
$self->throw("Unallowed parameter: $attr !") unless $OK_FIELD{$attr};
$self->{$attr} = shift if @_;
return $self->{$attr};
}
=head2 version
Title : version
Usage : exit if $prog->version() < 1.8
Function: Determine the version number of the program
Example :
Returns : float or undef
Args : none
=cut
sub version {
my ($self) = @_;
return undef unless $self->executable;
my $prog = $self->executable;
my $string = `$prog -version`;
if( $string =~ /Version:\s+\$\s*Name:\s+(\S+)\s+\$/ ) {
return $1;
} elsif( $string =~ /(Version *)/i ) {
return $1;
} else {
return undef;
}
}
=head2 predict_genes
Title : predict_genes
Usage : DEPRECATED. Use $factory->run($seq1,$seq2)
Function: Predict genes
Returns : A Bio::Seqfeature::Gene:GeneStructure object
Args : Name of a file containing a set of 2 fasta sequences in the order of
peptide and genomic sequences
or else 2 Bio::Seq objects.
Throws an exception if argument is not either a string (eg a filename)
or 2 Bio::Seq objects. If arguments are strings, throws exception if
file corresponding to string name can not be found.
=cut
sub predict_genes {
return shift->run(@_);
}
=head2 run
Title : run
Usage : 2 sequence objects
$genes = $factory->run($seq1, $seq2);
Function: run
Returns : A Bio::Seqfeature::Gene:GeneStructure object
Args : Names of a files each containing a fasta sequence in the order
of either (peptide sequence, genomic sequence) or (profile HMM,
genomic sequence). Alternatively any of the fasta sequence
filenames may be substituted with a Bio::Seq object.
Throws an exception if argument is not either a string (eg a filename)
or Bio::Seq objects. If arguments are strings, throws exception if
file corresponding to string name can not be found. Also throws an
exception if a profile HMM is expected (the -hmmer genewise switch has
been set).
=cut
sub run{
my ($self, $seq1, $seq2) = @_;
my ($attr, $value, $switch);
$self->io->_io_cleanup();
# Create input file pointer
my ($infile1,$infile2)= $self->_setinput($seq1, $seq2);
if (!($infile1 && $infile2)) {$self->throw("Bad input data (sequences need an id ) ");}
# run genewise
my @genes = $self->_run($infile1,$infile2);
return @genes;
}
=head2 _run
Title : _run
Usage : Internal function, not to be called directly
Function: Makes actual system call to a genewise program
Example :
Returns : L<Bio::SeqFeature::Gene::GeneStructure>
Args : Name of a files containing 2 sequences in the order of peptide and genomic
=cut
sub _run {
my ($self,$infile1,$infile2) = @_;
my $instring;
$self->debug("Program ".$self->executable."\n");
unless ( $self->executable ) {
$self->throw("Cannot run Genewise unless the executable is found. Check your environment variables or make sure genewise is in your path.");
}
my $paramstring = $self->_setparams;
my $commandstring = $self->executable." $paramstring $infile1 $infile2";
# this is to capture STDERR messages which leak out when you run programs
# with open(FH, "... |");
if (($self->silent && $self->quiet) &&
($^O !~ /os2|dos|amigaos/)) {
my $null = ($^O =~ m/mswin/i) ? 'NUL' : '/dev/null';
$commandstring .= " 2> $null";
}
my ($tfh1,$outfile1) = $self->io->tempfile(-dir=>$self->tempdir);
$self->debug("genewise command = $commandstring");
my $status = system("$commandstring > $outfile1");
$self->throw("Genewise call $commandstring crashed: $? \n") unless $status == 0;
my $genewiseParser = Bio::Tools::Genewise->new(-file=> $outfile1);
my @genes;
while (my $gene = $genewiseParser->next_prediction()) {
push @genes, $gene;
}
close ($tfh1);
undef ($tfh1);
return @genes;
}
sub get_strand {
my ($self,$start,$end) = @_;
$start || $self->throw("Need a start");
$end || $self->throw("Need an end");
my $strand;
if ($start > $end) {
my $tmp = $start;
$start = $end;
$end = $tmp;
$strand = -1;
}
else {
$strand = 1;
}
return ($start,$end,$strand);
}
sub _setinput {
my ($self, $arg1, $seq2) = @_;
my ($tfh1,$tfh2,$outfile1,$outfile2);
$self->throw("calling with not enough arguments") unless $arg1 && $seq2;
# Not going to set _query_pep/_subject_dna_seq if you pass in a
# filename
unless( ref($arg1) ) {
unless( -e $arg1 ) {
if ($self->hmmer) {
$self->throw("Argument1 was not a HMMER profile HMM file\n")
}
else {
$self->throw("Argument1 is not a Bio::PrimarySeqI object nor file\n");
}
}
$outfile1 = $arg1;
}
else {
if ($self->hmmer) {
$self->throw("Argument1 was not a HMMER profile HMM file\n")
}
else {
($tfh1,$outfile1) = $self->io->tempfile(-dir=>$self->tempdir);
my $out1 = Bio::SeqIO->new('-fh' => $tfh1,
'-format' => 'fasta');
$out1->write_seq($arg1);
$self->_query_pep_seq($arg1);
# Make sure you close things - this is what creates
# Out of filehandle errors
close($tfh1);
undef $tfh1;
}
}
unless( ref($seq2) ) {
unless( -e $seq2 ) {
$self->throw("Sequence2 is not a Bio::PrimarySeqI object nor file\n");
}
$outfile2 = $seq2;
}
else {
($tfh2,$outfile2) = $self->io->tempfile(-dir=>$self->tempdir);
my $out2 = Bio::SeqIO->new('-fh' => $tfh2,
'-format' => 'fasta');
$out2->write_seq($seq2);
$self->_subject_dna_seq($seq2);
# Make sure you close things - this is what creates
# Out of filehandle errors
close($tfh2);
undef $tfh2;
}
return ($outfile1,$outfile2);
}
=head2 _setparams
Title : _setparams
Usage : Internal function, not to be called directly
Function: creates a string of params to be used in the command string
Example :
Returns : string of params
Args :
=cut
sub _setparams {
my ($self) = @_;
my $param_string;
foreach my $attr(@GENEWISE_PARAMS){
my $value = $self->$attr();
next unless (defined $value);
my $attr_key = ' -'.(lc $attr);
$param_string .= $attr_key.' '.$value;
}
foreach my $attr(@GENEWISE_SWITCHES){
my $value = $self->$attr();
next unless (defined $value);
my $attr_key = ' -'.(lc $attr);
$param_string .=$attr_key;
}
$param_string = $param_string." -genesf"; #specify the output option
return $param_string;
}
=head2 _query_pep_seq
Title : _query_pep_seq
Usage : Internal function, not to be called directly
Function: get/set for the query sequence
Example :
Returns :
Args :
=cut
sub _query_pep_seq {
my ($self,$seq) = @_;
if(defined $seq){
$self->{'_query_pep_seq'} = $seq;
}
return $self->{'_query_pep_seq'};
}
=head2 _subject_dna_seq
Title : _subject_dna_seq
Usage : Internal function, not to be called directly
Function: get/set for the subject sequence
Example :
Returns :
Args :
=cut
sub _subject_dna_seq {
my ($self,$seq) = @_;
if(defined $seq){
$self->{'_subject_dna_seq'} = $seq;
}
return $self->{'_subject_dna_seq'};
}
1;