#
# Please direct questions and support issues to <bioperl-l@bioperl.org>
#
# Cared for by Tania Oh
#
# Copyright Tania Oh
#
# You may distribute this module under the same terms as perl itself
# POD documentation - main docs before the code
=head1 NAME
Bio::Tools::Run::Eponine - Object for execution of the Eponine which
is a mammalian TSS predictor
=head1 SYNOPSIS
use Bio::Tools::Run::Eponine;
use strict;
my $seq = "/data/seq.fa";
my $threshold = "0.999";
my @params = ( '-seq' => $seq,
'-threshold' => $threshold,
'-epojar' => '/usr/local/bin/eponine-scan.jar',
'-java' => '/usr/local/bin/java');
my $factory = Bio::Tools::Run::Eponine->new(@params);
# run eponine against fasta
my $r = $factory->run($seq);
my $parser = Bio::Tools::Eponine->new($r);
while (my $feat = $parser->next_prediction){
#$feat contains array of SeqFeature
foreach my $orf($feat){
print $orf->seqname. "\n";
}
}
# Various additional options and input formats are available. See
# the DESCRIPTION section for details.
=head1 DESCRIPTION
wrapper for eponine, a mammalian TSS predictor.
The environment variable EPONINEDIR must be set to point at either the
directory which contains eponine-scan.jar or directly at the jar which
eponine-scan classfiles. NOTE: EPONINEDIR must point at the real file
not a symlink.
=head1 FEEDBACK
=head2 Mailing Lists
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to one
of the Bioperl mailing lists. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/wiki/Mailing_lists - About the mailing lists
=head2 Support
Please direct usage questions or support issues to the mailing list:
I<bioperl-l@bioperl.org>
rather than to the module maintainer directly. Many experienced and
reponsive experts will be able look at the problem and quickly
address it. Please include a thorough description of the problem
with code and data examples if at all possible.
=head2 Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution. Bug reports can be submitted via the
web:
http://redmine.open-bio.org/projects/bioperl/
=head1 AUTHOR
Email gisoht@nus.edu.sg
=head1 APPENDIX
The rest of the documentation details each of the object
methods. Internal methods are usually preceded with a _
=cut
package Bio::Tools::Run::Eponine;
#tgot to take inmore parameters
use vars qw($AUTOLOAD @ISA @EPONINE_PARAMS %EPONINE_PARAMS
$EPOJAR $JAVA $PROGRAMDIR $PROGRAMNAME $PROGRAM
$TMPDIR $TMPOUTFILE $DEFAULT_THRESHOLD
%OK_FIELD);
use strict;
use Bio::Tools::Eponine;
use Bio::Root::Root;
use Bio::Root::IO;
use Bio::Tools::Run::WrapperBase;
@ISA = qw(Bio::Root::Root Bio::Tools::Run::WrapperBase);
BEGIN {
$DEFAULT_THRESHOLD = 50;
$PROGRAMNAME = 'java';
$EPOJAR = 'eponine-scan.jar';
if( ! defined $PROGRAMDIR ) {
$PROGRAMDIR = $ENV{'JAVA_HOME'} || $ENV{'JAVA_DIR'};
}
if (defined $PROGRAMDIR) {
foreach my $progname ( [qw(java)],[qw(bin java)] ) {
my $f = Bio::Root::IO->catfile($PROGRAMDIR, @$progname);
if( -e $f && -x $f ) {
$PROGRAM = $f;
last;
}
}
}
if( $ENV{'EPONINEDIR'} ) {
if ( -d $ENV{'EPONINEDIR'} ) {
$EPOJAR = Bio::Root::IO->catfile($ENV{'EPONINEDIR'}, $EPOJAR)
} elsif(-e $ENV{'EPONINEDIR'}) {
$EPOJAR = $ENV{'EPONINEDIR'};
}
if ( ! -e $EPOJAR) {
$EPOJAR =undef;
}
}
%EPONINE_PARAMS = ('SEQ' => '/tmp/test.fa',
'THRESHOLD' => '0.999',
'EPOJAR' => '/usr/local/bin/eponine-scan.jar',
'JAVA' => '/usr/java/jre1.3.1_02/bin/java');
@EPONINE_PARAMS=qw(SEQ THRESHOLD JAVA EPOJAR);
foreach my $attr ( @EPONINE_PARAMS)
{ $OK_FIELD{$attr}++; }
}
sub AUTOLOAD {
my $self = shift;
my $attr = $AUTOLOAD;
$self->debug( "************ attr: $attr\n");
$attr =~ s/.*:://;
$attr = uc $attr;
$self->throw("Unallowed parameter: $attr !") unless $OK_FIELD{$attr};
$self->{$attr} = shift if @_;
return $self->{$attr};
}
sub new {
my ($caller, @args) = @_;
# chained new
my $self = $caller->SUPER::new(@args);
# so that tempfiles are cleaned up
my $java;
my $seq;
my $threshold;
my $epojar;
my ($attr, $value);
($TMPDIR) = $self->tempdir(CLEANUP=>1);
my $tfh;
($tfh,$TMPOUTFILE) = $self->io->tempfile(-dir => $TMPDIR);
close($tfh);
undef $tfh;
while (@args) {
$attr = shift @args;
$value = shift @args;
next if( $attr =~ /^-/ ); # don't want named parameters
if ($attr =~/JAVA/i) {
$java = $value;
next;
}
if ($attr =~ /EPOJAR/i){
$epojar = $value;
next;
}
if ($attr =~ /THRESHOLD/i){
$threshold = $value;
next;
}
if ($attr =~ /SEQ/i){
$seq = $value;
next;
}
$self->$attr($value);
}
$self->{'_java'} = undef; # location of java vm
$self->{'_epojar'} = undef; # location of eponine-scan.jar executable JAR file.
$self->{'_threshold'} = 0.999; # minimum posterior for filtering predictions
$self->{'_filename'} = undef; #location of seq
$seq = $EPONINE_PARAMS{'seq'} unless defined $seq;
$threshold = $EPONINE_PARAMS{'threshold'} unless defined $threshold;
if (! defined $epojar && defined $EPOJAR) {
$epojar = $EPOJAR;
}
else {
$epojar = $EPONINE_PARAMS{'epojar'} unless defined $epojar;
}
if (! defined $java && defined $PROGRAM) {
$java = $PROGRAM;
}
else {
$java = $EPONINE_PARAMS{'JAVA'} unless defined $java;
}
$self->filename($seq) if ($seq);
if (-x $java) {
# full path assumed
$self->java($java);
}
$self->epojar($epojar) if (defined $epojar);
if (defined $threshold && $threshold >=0 ){
$self->threshold($threshold);
} else {
$self->threshold($DEFAULT_THRESHOLD);
}
return $self;
}
=head2 java
Title : java
Usage : $obj->java('/usr/opt/java130/bin/java');
Function: Get/set method for the location of java VM
Args : File path (optional)
=cut
sub executable { shift->java(@_); }
sub java {
my ($self, $exe,$warn) = @_;
if( defined $exe ) {
$self->{'_pathtojava'} = $exe;
}
unless( defined $self->{'_pathtojava'} ) {
if( $PROGRAM && -e $PROGRAM && -x $PROGRAM ) {
$self->{'_pathtojava'} = $PROGRAM;
} else {
my $exe;
if( ( $exe = $self->io->exists_exe($PROGRAMNAME) ) &&
-x $exe ) {
$self->{'_pathtojava'} = $exe;
} else {
$self->warn("Cannot find executable for $PROGRAMNAME") if $warn;
$self->{'_pathtojava'} = undef;
}
}
}
$self->{'_pathtojava'};
}
=head2 epojar
Title : epojar
Usage : $obj->epojar('/some/path/to/eponine-scan.jar');
Function: Get/set method for the location of the eponine-scan executable JAR
Args : Path (optional)
=cut
sub epojar {
my ($self, $location) = @_;
if ($location)
{
unless( $location ) {
$self->warn("eponine-scan.jar not found at $location: $!\n");
return;
}
$self->{'_epojar'} = $location ;
}
return $self->{'_epojar'};
}
=head2 threshold
Title : threshold
Usage : my $threshold = $self->threshold
Function: Get/Set the threshold for Eponine
Returns : string
Args : b/w 0.9 and 1.0
=cut
sub threshold{
my ($self, $threshold) = @_;
if (defined $threshold) {
$self->{'_threshold'} = $threshold ;
}
return $self->{'_threshold'};
}
=head2 run
Title : run
Usage : my @genes = $self->run($seq)
Function: runs Eponine and creates an array of features
Returns : An Array of SeqFeatures
Args : A Bio::PrimarySeqI
=cut
sub run{
my ($self,$seq) = @_;
my $infile = $self->_setinput($seq);
my @tss = $self->_run_eponine($infile);
return @tss;
}
=head2 predict_TSS
Title : predict_TSS
Usage : Alias for run()
=cut
sub predict_TSS {
return shift->run(@_);
}
=head2 _setinput()
Title : _setinput
Usage : Internal function, not to be called directly
Function: writes input sequence to file and return the file name
Example :
Returns : string
Args :
=cut
sub _setinput {
my ($self,$seq) = @_;
#better be a file
if(!ref $seq){
return $seq;
}
my ($tfh1,$inputfile) = $self->tempfile(-dir=>$TMPDIR);
my $in = Bio::SeqIO->new(-fh=> $tfh1 , '-format' => 'Fasta');
$in->write_seq($seq);
close($tfh1);
undef $tfh1;
return ($inputfile);
}
=head2 _run_eponine
Title : run_eponine
Usage : $obj->_run_eponine()
Function: execs the Java VM to run eponine
Returns : none
Args : none
=cut
sub _run_eponine {
my ($self,$infile) = @_;
my $result = $TMPOUTFILE;
my @tss;
#run eponine
$self->debug( "Running eponine-scan\n");
my ($java,$epojar) = ( $self->java,
$self->epojar);
unless( defined $java && -e $java && -x $java ) {
$self->warn("Cannot find java");
return;
}
if (! defined $epojar) { $self->warn("Don't know the name of the Eponine jar file"); return; }
if (! -e $epojar) {
$self->warn("Cannot find Eponine jar: $epojar - either you specified an incorrect path in\nEPONINEDIR or it was not in the current working directory");
return;
}
my $cmd = $self->java.' -jar '.$self->epojar.' -seq '.$infile.' -threshold '.$self->threshold." > ".$result;
$self->throw("Error running eponine-scan on ".$self->filename.
" \n Check your java version, it has to be version 1.2 or later. Eponine crashed ($cmd) crashed: $? \n")
if (system ($cmd));
#parse results even though it's wierd.. thought parser and wrapper should be separate
my $epoParser = Bio::Tools::Eponine->new(-file =>$result);
while (my $tss = $epoParser->next_prediction()){
push (@tss, $tss);
}
return @tss;
}
1;
__END__