# Build a FastTree factory
  $factory = Bio::Tools::Run::Phylo::FastTree->new(-quiet => 1,
                                                   -fastest => 1);
  # Get an alignment
  my $alignio = Bio::AlignIO->new(
        -format => 'fasta',
        -file   => '219877.cdna.fasta');
  my $alnobj = $alignio->next_aln;

  # Analyze the aligment and get a Tree
  my $tree = $factory->run($alnobj);


Get a Bio::Tree object given a protein or DNA alignment.


Mailing Lists

User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated.                  - General discussion  - About the mailing lists


Please direct usage questions or support issues to the mailing list:

Do not contact the module maintainer directly. Many experienced experts at bioperl-l will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.

Reporting Bugs

Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web:

AUTHOR - Brian Osborne



The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _


 Title   : new
 Usage   : my $treebuilder = Bio::Tools::Run::Phylo::FastTree->new();
 Function: Constructor
 Returns : Bio::Tools::Run::Phylo::FastTree
 Args    : -outfile_name => $outname


 Title   : program_name
 Usage   : $factory->program_name()
 Function: holds the program name
 Returns:  string
 Args    : None


 Title   : program_dir
 Usage   : $factory->program_dir(@params)
 Function: returns the program directory
 Returns:  string
 Args    :


 Title   : error_string
 Usage   : $obj->error_string($newval)
 Function: Where the output from the last analysus run is stored.
 Returns : value of error_string
 Args    : newvalue (optional)


 Title   : version
 Usage   : exit if $prog->version() < 1.8
 Function: Determine the version number of the program
 Example :
 Returns : float or undef
 Args    : none


 Title   : run
 Usage   : $factory->run($stockholm_file) OR
 Function: Runs FastTree to generate a tree 
 Returns : Bio::Tree::Tree object
 Args    : File name for your input alignment in stockholm format, OR
           Bio::Align::AlignI compliant object (eg. Bio::SimpleAlign).


 Title   : _run
 Usage   : Internal function, not to be called directly
 Function: Runs the application
 Returns : Tree object
 Args    : Alignment file name


 Title   : _write_alignfile
 Usage   : Internal function, not to be called directly
 Function: Create an alignment file
 Returns : filename
 Args    : Bio::Align::AlignI


 Title   : _alphabet
 Usage   : my $alphabet = $self->_alphabet;
 Function: Get the alphabet of the input alignment, defaults to 'dna'
 Returns : 'dna' or 'protein'
 Args    : Alignment file


 Title   :  _setparams
 Usage   :  Internal function, not to be called directly    
 Function:  Create parameter inputs for FastTree program
 Example :
 Returns : parameter string to be passed to FastTree
 Args    : name of calling object

Bio::Tools::Run::BaseWrapper methods


 Title   : no_param_checks
 Usage   : $obj->no_param_checks($newval)
 Function: Boolean flag as to whether or not we should
           trust the sanity checks for parameter values  
 Returns : value of no_param_checks
 Args    : newvalue (optional)


 Title   : save_tempfiles
 Usage   : $obj->save_tempfiles($newval)
 Returns : value of save_tempfiles
 Args    : newvalue (optional)


 Title   : outfile_name
 Usage   : my $outfile = $FastTree->outfile_name();
 Function: Get/Set the name of the output file for this run
           (if you wanted to do something special)
 Returns : string
 Args    : [optional] string to set value to


 Title   : tempdir
 Usage   : my $tmpdir = $self->tempdir();
 Function: Retrieve a temporary directory name (which is created)
 Returns : string which is the name of the temporary directory
 Args    : none


 Title   : cleanup
 Usage   : $FastTree->cleanup();
 Function: Will cleanup the tempdir directory
 Returns : none
 Args    : none


 Title   : io
 Usage   : $obj->io($newval)
 Function:  Gets a L<Bio::Root::IO> object
 Returns : L<Bio::Root::IO>
 Args    : none